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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated ADH4 Monoclonal anti (CL488-67303)

    IF Staining of HepG2 using CL488-67303

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 ADH4 antibody (CL488-67303, Clone: 3D12E9 ) at dilution of 1:200.

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CoraLite® Plus 488-conjugated ADH4 Monoclonal antibody

ADH4 Monoclonal Antibody for IF/ICC
Cat No. CL488-67303
Clone No.3D12E9

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Synonyms

ADH 2, ADH4, Alcohol dehydrogenase 4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67303 targets ADH4 in IF/ICC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ADH4 fusion protein Ag9884 相同性解析による交差性が予測される生物種
Full Name alcohol dehydrogenase 4 (class II), pi polypeptide
Calculated molecular weight 380 aa, 40 kDa
Observed molecular weight 40-43 kDa
GenBank accession numberBC022319
Gene Symbol ADH4
Gene ID (NCBI) 127
RRIDAB_2934532
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08319
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This gene encodes the class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. ADH4 plays a critical role in alcoholism development and alcohol toxicology and is also associated with the development of esophageal cancer(PMID:33556337, 26039424, 24051444, 22147505).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ADH4 antibody CL488-67303Download protocol
Standard Protocols
Click here to view our Standard Protocols