- Featured Product
- KD/KO Validated
Adiponectin receptor 1 Monoclonal antibody
Adiponectin receptor 1 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (3)
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1F12B3
Cat no : 66619-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, L02 cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
Product Information
66619-1-Ig targets Adiponectin receptor 1 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Adiponectin receptor 1 fusion protein Ag18187 相同性解析による交差性が予測される生物種 |
| Full Name | adiponectin receptor 1 |
| Calculated molecular weight | 375 aa, 43 kDa |
| Observed molecular weight | 43 kDa |
| GenBank accession number | BC010743 |
| Gene symbol | Adiponectin receptor 1 |
| Gene ID (NCBI) | 51094 |
| RRID | AB_2881979 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Adiponectin is a hormone secreted by adipocytes that acts as an antidiabetic and anti-atherogenic adipokine. Two receptors for adiponectin have been identified: adiponectin receptor 1 (AdipoR1) and adiponectin receptor 2 (AdipoR2). They are seven-transmembrane domain receptors and mediate increased AMPK, MAPK and PPARA ligand activity in response to adiponectin. AdipoR1 is found in many tissues, being particularly abundant in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver (PMID: 24417942). AdipoR1 has a high affinity for globular adiponectin and a very low affinity for full-length adiponectin. AdipoR2 has an intermediate affinity for both forms. The human ADIPOR1 gene maps to chromosome 1q32.1, and encodes a 375-amino acid protein with a molecular weight of 43 kDa. This antibody was raised against 1-72aa of human AdipoR1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Adiponectin receptor 1 antibody 66619-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Food Funct A flavonoid-rich Smilax china L. extract prevents obesity by upregulating the adiponectin-receptor/AMPK signalling pathway and modulating the gut microbiota in mice. | ||
Food Funct Quercetin regulates skeletal muscle fiber type switching via adiponectin signaling. | ||
Biol Open A stem cell based in vitro model of NAFLD enables the analysis of patient specific individual metabolic adaptations in response to a high fat diet and AdipoRon interference. | ||
Anim Biotechnol Leucine regulates porcine muscle fiber type transformation via adiponectin signaling pathway. | ||
Anim Biotechnol Effect of dietary leucine supplementation on skeletal muscle fiber type transformation in weaning piglets | ||
J Agric Food Chem Probiotic Yogurt Alleviates High-Fat Diet-Induced Lipid Accumulation and Insulin Resistance in Mice via the Adiponectin Pathway |
