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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ADPGK Monoclonal antibody (68034-1-Ig)

    WB analysis using 68034-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of LNCaP cells using ADPGK Monoclonal antibody (68034-1-Ig)

    WB analysis of LNCaP using 68034-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using ADPGK Monoclonal antibody (68034-1-Ig)

    WB analysis of A549 using 68034-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ADPGK Monoclonal antibody (68034-1-Ig)

    WB analysis of HeLa using 68034-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using ADPGK Monoclonal antibody (68034-1-Ig)

    WB analysis of THP-1 using 68034-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using ADPGK Monoclonal antibody (68034-1-Ig)

    WB analysis of human placenta using 68034-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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ADPGK Monoclonal antibody

ADPGK Monoclonal Antibody for WB, ELISA
Cat No. 68034-1-Ig
Clone No.2B4B8

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Synonyms

2610017G09Rik, ADP dependent glucokinase, ADP GK, ADPGK, DKFZp434B195, RbBP 35

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HeLa cells, human placenta tissue, LNCaP cells, THP-1 cells, Jurkat cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68034-1-Ig targets ADPGK in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ADPGK fusion protein Ag31242 相同性解析による交差性が予測される生物種
Full Name ADP-dependent glucokinase
Calculated molecular weight 497 aa, 54 kDa
Observed molecular weight51 kDa
GenBank accession numberBC006112
Gene Symbol ADPGK
Gene ID (NCBI) 83440
RRIDAB_2918776
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BRR6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADP-dependent glucokinase (ADPGK) has frst been described 1994 in hyperthermophilic archaea as a novel glucose-phosphorylating enzyme dependent on ADP (adenosine diphosphate) instead of ATP (adenosine triphosphate). Highest ADPGK expression is found in immune cells of both myeloid and lymphoid lineages. Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency (By similarity).

Protocols

Product Specific Protocols
WB protocol for ADPGK antibody 68034-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols