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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ADPGK Monoclonal antibody (68034-1-Ig)

    WB analysis using 68034-1-Ig (same clone as 68034-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68034-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of LNCaP cells using ADPGK Monoclonal antibody (68034-1-Ig)

    WB analysis of LNCaP using 68034-1-Ig (same clone as 68034-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68034-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using ADPGK Monoclonal antibody (68034-1-Ig)

    WB analysis of A549 using 68034-1-Ig (same clone as 68034-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68034-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ADPGK Monoclonal antibody (68034-1-Ig)

    WB analysis of HeLa using 68034-1-Ig (same clone as 68034-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68034-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using ADPGK Monoclonal antibody (68034-1-Ig)

    WB analysis of THP-1 using 68034-1-Ig (same clone as 68034-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68034-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using ADPGK Monoclonal antibody (68034-1-Ig)

    WB analysis of human placenta using 68034-1-Ig (same clone as 68034-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68034-1-Ig (ADPGK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68034-1-PBS in a different storage buffer formulation.

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ADPGK Monoclonal antibody, PBS Only

ADPGK Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68034-1-PBS
Clone No.2B4B8

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Synonyms

2610017G09Rik, ADP dependent glucokinase, ADP GK, ADPGK, DKFZp434B195, RbBP 35

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68034-1-PBS targets ADPGK in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ADPGK fusion protein Ag31242 相同性解析による交差性が予測される生物種
Full Name ADP-dependent glucokinase
Calculated molecular weight 497 aa, 54 kDa
Observed molecular weight51 kDa
GenBank accession numberBC006112
Gene Symbol ADPGK
Gene ID (NCBI) 83440
RRIDAB_2918776
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BRR6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ADP-dependent glucokinase (ADPGK) has frst been described 1994 in hyperthermophilic archaea as a novel glucose-phosphorylating enzyme dependent on ADP (adenosine diphosphate) instead of ATP (adenosine triphosphate). Highest ADPGK expression is found in immune cells of both myeloid and lymphoid lineages. Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency (By similarity).