ADRBK1 Monoclonal antibody
ADRBK1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human and More (2)
Applications
WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
2F7A5
Cat no : 67067-1-Ig
Synonyms
Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | THP-1 cells, U-937 cells, HL-60 cells, K-562 cells, Jurkat cells |
| Positive IHC detected in | human lymphoma tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Jurkat cells |
| Positive FC (Intra) detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
67067-1-Ig targets ADRBK1 in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ADRBK1 fusion protein Ag5071 相同性解析による交差性が予測される生物種 |
| Full Name | adrenergic, beta, receptor kinase 1 |
| Calculated molecular weight | 689 aa, 80 kDa |
| Observed molecular weight | 80 kDa |
| GenBank accession number | BC037963 |
| Gene symbol | ADRBK1 |
| Gene ID (NCBI) | 156 |
| RRID | AB_2882377 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ADRBK1 antibody 67067-1-Ig | Download protocol |
| IHC protocol for ADRBK1 antibody 67067-1-Ig | Download protocol |
| IF protocol for ADRBK1 antibody 67067-1-Ig | Download protocol |
| FC protocol for ADRBK1 antibody 67067-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Phytother Res Paeoniflorin-6'-O-benzene sulfonate protected the intestinal epithelial barrier by restoring the inhibitory effect of GRK2 and β-arrestin 2 on ERK1/2-NF-κB |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Charlotte (Verified Customer) (08-05-2024) | I tested the antibody in several cells (here shown RPE1 cells). The GRK2 signal (Cy3) versus the nuclei (Dapi).
 |
