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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ADRBK1 Monoclonal antibody

ADRBK1 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (2)

Applications

WB, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

2F7A5

Cat no : 67067-1-Ig

Synonyms

ADRBK1, BARK, BARK1, Beta ARK 1, BETA ARK1, FLJ16718, GRK2

Validation Data Gallery

Applications:
  • WB analysis using 67067-1-Ig

    WB analysis using 67067-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67067-1-Ig (ADRBK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of U-937 using 67067-1-Ig

    WB analysis of U-937 using 67067-1-Ig

    U-937 cells were subjected to SDS PAGE followed by western blot with 67067-1-Ig (ADRBK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 67067-1-Ig

    IHC staining of human lymphoma using 67067-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67067-1-Ig (ADRBK1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lymphoma using 67067-1-Ig

    IHC staining of human lymphoma using 67067-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67067-1-Ig (ADRBK1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 67067-1-Ig

    IHC staining of human tonsillitis using 67067-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67067-1-Ig (ADRBK1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 67067-1-Ig

    IHC staining of human tonsillitis using 67067-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67067-1-Ig (ADRBK1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 67067-1-Ig

    IHC staining of human tonsillitis using 67067-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67067-1-Ig (ADRBK1 antibody) at dilution of 1:400 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 67067-1-Ig

    IHC staining of human tonsillitis using 67067-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67067-1-Ig (ADRBK1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Jurkat using 67067-1-Ig

    IF Staining of Jurkat using 67067-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ADRBK1 antibody (67067-1-Ig, Clone: 2F7A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of HeLa using 67067-1-Ig

    FC experiment of HeLa using 67067-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ADRBK1 (67067-1-Ig, Clone:2F7A5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inTHP-1 cells, U-937 cells, HL-60 cells, K-562 cells, Jurkat cells
Positive IHC detected inhuman lymphoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inJurkat cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

67067-1-Ig targets ADRBK1 in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ADRBK1 fusion protein Ag5071 相同性解析による交差性が予測される生物種
Full Name adrenergic, beta, receptor kinase 1
Calculated molecular weight 689 aa, 80 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC037963
Gene symbol ADRBK1
Gene ID (NCBI) 156
RRIDAB_2882377
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ADRBK1 antibody 67067-1-IgDownload protocol
IHC protocol for ADRBK1 antibody 67067-1-IgDownload protocol
IF protocol for ADRBK1 antibody 67067-1-IgDownload protocol
FC protocol for ADRBK1 antibody 67067-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF,CoIP

Phytother Res

Paeoniflorin-6'-O-benzene sulfonate protected the intestinal epithelial barrier by restoring the inhibitory effect of GRK2 and β-arrestin 2 on ERK1/2-NF-κB

Authors - Mengya Jiang
View Article