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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated ADRBK1 Monoclonal antibody

ADRBK1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2F7A5

Cat no : CL594-67067

Synonyms

ADRBK1, BARK, BARK1, Beta ARK 1, BETA ARK1, FLJ16718, GRK2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CoraLite®594-conjugated ADRBK1 Monoclonal antibody (CL594-67067)

    IF Staining of Jurkat using CL594-67067

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite®594 ADRBK1 antibody (CL594-67067, Clone: 2F7A5 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated ADRBK1 Monoclonal antibody (CL594-67067)

    FC experiment of HeLa using CL594-67067

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human ADRBK1 (CL594-67067, Clone:2F7A5) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67067 targets ADRBK1 in IF/ICC, FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ADRBK1 fusion protein Ag5071 相同性解析による交差性が予測される生物種
Full Name adrenergic, beta, receptor kinase 1
Calculated molecular weight 689 aa, 80 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC037963
Gene symbol ADRBK1
Gene ID (NCBI) 156
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL594 ADRBK1 antibody CL594-67067Download protocol
FC protocol for CL594 ADRBK1 antibody CL594-67067Download protocol
Standard Protocols
Click here to view our Standard Protocols