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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Perilipin-2 Recombinant antibody

Perilipin-2 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230274F4

Cat no : 80362-2-RR

Synonyms

ADRP/Perilipin 2, ADRP, ADFP, Perilipin 2, 230274F4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ADRP Recombinant antibody (80362-2-RR)

    WB analysis using 80362-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80362-2-RR (ADRP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ADRP Recombinant antibody (80362-2-RR)

    WB analysis using 80362-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80362-2-RR (ADRP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ADRP Recombinant antibody (80362-2-RR)

    IHC staining of mouse liver using 80362-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 80362-2-RR (ADRP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ADRP Recombinant antibody (80362-2-RR)

    IHC staining of mouse liver using 80362-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 80362-2-RR (ADRP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ADRP Recombinant antibody (80362-2-RR)

    IF Staining of HeLa using 80362-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using ADRP antibody (80362-2-RR, Clone: 230274F4 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ADRP Recombinant antibody (80362-2-RR)

    IF Staining of HeLa using 80362-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using ADRP antibody (80362-2-RR, Clone: 230274F4 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Perilipin-2 Recombinant antibody (80362-2-RR)

    IF Staining of HeLa using 80362-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using ADRP antibody (80362-2-RR, Clone: 230274F4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HepG2 cells using Perilipin-2 Recombinant antibody (80362-2-RR)

    FC experiment of HepG2 using 80362-2-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ADRP Recombinant antibody (80362-2-RR, Clone:230274F4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 80362-2-RR

    Affinity and Kinetic Characterization of 80362-2-RR

    Biolayer interferometry (BLl) kinetic assays of 80362-2-RR against Human ADRP were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inTHP-1 cells, HepG2 cells, K-562 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inoleic acid treated HeLa cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80362-2-RR targets Perilipin-2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Perilipin-2 fusion protein Ag7539 相同性解析による交差性が予測される生物種
Full Name adipose differentiation-related protein
Calculated molecular weight 48 kDa
Observed molecular weight48 kDa
GenBank accession numberBC005127
Gene symbol Perilipin-2
Gene ID (NCBI) 123
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases.

Protocols

Product Specific Protocols
WB protocol for Perilipin-2 antibody 80362-2-RRDownload protocol
IHC protocol for Perilipin-2 antibody 80362-2-RRDownload protocol
IF protocol for Perilipin-2 antibody 80362-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols