Perilipin-2 Recombinant antibody
Perilipin-2 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
230274F4
Cat no : 80362-2-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | THP-1 cells, HepG2 cells, K-562 cells |
Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | oleic acid treated HeLa cells |
Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
80362-2-RR targets Perilipin-2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Perilipin-2 fusion protein Ag7539 相同性解析による交差性が予測される生物種 |
Full Name | adipose differentiation-related protein |
Calculated molecular weight | 48 kDa |
Observed molecular weight | 48 kDa |
GenBank accession number | BC005127 |
Gene symbol | Perilipin-2 |
Gene ID (NCBI) | 123 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Perilipin-2 antibody 80362-2-RR | Download protocol |
IHC protocol for Perilipin-2 antibody 80362-2-RR | Download protocol |
IF protocol for Perilipin-2 antibody 80362-2-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |