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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated ADRP Recombinant antibody

ADRP Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230274F4

Cat no : CL488-80362-2

Synonyms

Perilipin 2, ADRP/Perilipin 2, ADFP, Perilipin-2, 230274F4

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated ADRP Recombinant ant (CL488-80362-2)

    IF Staining of HeLa using CL488-80362-2

    Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using CoraLite® Plus 488 ADRP antibody (CL488-80362-2, Clone: 230274F4 ) at dilution of 1:200, CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated ADRP Recombinant ant (CL488-80362-2)

    FC experiment of HepG2 using CL488-80362-2

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 ADRP Recombinant Antibody (CL488-80362-2, Clone:230274F4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inoleic acid treated HeLa cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-80362-2 targets ADRP in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ADRP fusion protein Ag7539 相同性解析による交差性が予測される生物種
Full Name adipose differentiation-related protein
Calculated molecular weight 48 kDa
Observed molecular weight48 kDa
GenBank accession numberBC005127
Gene symbol Perilipin-2
Gene ID (NCBI) 123
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ADRP antibody CL488-80362-2Download protocol
Standard Protocols
Click here to view our Standard Protocols