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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AEG-1/MTDH Monoclonal antibody (68592-1-Ig)

    WB analysis using 68592-1-Ig (same clone as 68592-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68592-1-Ig (AEG-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68592-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using AEG-1/MTDH Monoclonal antibody (68592-1-Ig)

    IHC staining of human rectal cancer using 68592-1-Ig (same clone as 68592-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68592-1-Ig (AEG-1/MTDH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68592-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using AEG-1/MTDH Monoclonal antibody (68592-1-Ig)

    IHC staining of human rectal cancer using 68592-1-Ig (same clone as 68592-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68592-1-Ig (AEG-1/MTDH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68592-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using AEG-1/MTDH Monoclonal antibody (68592-1-Ig)

    IHC staining of human rectal cancer using 68592-1-Ig (same clone as 68592-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 68592-1-Ig (AEG-1/MTDH antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68592-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using AEG-1/MTDH Monoclonal antibody (68592-1-Ig)

    IHC staining of human tonsillitis using 68592-1-Ig (same clone as 68592-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68592-1-Ig (AEG-1/MTDH antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68592-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using AEG-1/MTDH Monoclonal antibody (68592-1-Ig)

    IF Staining of A431 using 68592-1-Ig (same clone as 68592-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using AEG-1/MTDH antibody (68592-1-Ig, Clone: 1D8C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68592-1-PBS in a different storage buffer formulation.

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AEG-1/MTDH Monoclonal antibody, PBS Only

AEG-1/MTDH Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68592-1-PBS
Clone No.1D8C2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

AEG-1, MTDH, 1D8C2, 3D3/LYRIC, AEG 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68592-1-PBS targets AEG-1/MTDH in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AEG-1/MTDH fusion protein Ag5033 相同性解析による交差性が予測される生物種
Full Name metadherin
Calculated molecular weight 582 aa, 64 kDa
Observed molecular weight70-80 kDa
GenBank accession numberBC045642
Gene Symbol AEG-1/MTDH
Gene ID (NCBI) 92140
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ86UE4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression.