CoraLite® Plus 488-conjugated AFM Monoclonal antibody
AFM Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2G9F6
Cat no : CL488-66113
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of CL488-66113 is AFM Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | AFM fusion protein Ag16866 相同性解析による交差性が予測される生物種 |
Full Name | afamin |
Calculated molecular weight | 599 aa, 69 kDa |
Observed molecular weight | 85 kDa |
GenBank accession number | BC109020 |
Gene symbol | AFM |
Gene ID (NCBI) | 173 |
RRID | AB_2883260 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AFM (Afamin) is a member of the albumin superfamily, which comprises albumin, vitamin D-binding protein, a-fetoprotein, and afamin. AFM is present in the plasma/serum, cerebrospinal fluid, and follicular fluid, and functions as a vitamin E-binding protein. Alterations of expression level of AFM have been linked to variety of diseases like cancer.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 AFM antibody CL488-66113 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |