AFP Monoclonal antibody
AFP Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
4B3E4
Cat no : 67852-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, HuH-7 cells, human testis tissue, human placenta tissue |
Positive IHC detected in | human liver tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
67852-1-Ig targets AFP in WB, IHC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | AFP fusion protein Ag6089 相同性解析による交差性が予測される生物種 |
Full Name | alpha-fetoprotein |
Calculated molecular weight | 69 kDa |
Observed molecular weight | 68-72 kDa |
GenBank accession number | BC027881 |
Gene symbol | AFP |
Gene ID (NCBI) | 174 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AFP (Alpha-fetoprotein) is a major plasma protein in the fetus and its concentration is very low in the adult (PMID:24120489). AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma, indicating that AFP can serve as a tumor marker (PMID: 18669658). AFP is also a glycosylated protein and based on its binding capability to lectin Lens Culinaris Agglutinin (LCA), and total AFP can be separated into three different glycoforms, AFP-L1, AFP-L2, and AFP-L3. Core-fucosylated form of AFP (AFP-L3) is a more specific indicator than total AFP for HCC (PMID: 33128033, 35458505)
Protocols
Product Specific Protocols | |
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WB protocol for AFP antibody 67852-1-Ig | Download protocol |
IHC protocol for AFP antibody 67852-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |