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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AFP Monoclonal antibody, PBS Only

AFP Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4B3E4

Cat no : 67852-1-PBS

Synonyms

AFP, Alpha 1 fetoprotein, Alpha fetoglobulin, alpha fetoprotein, FETA, HPAFP

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using AFP Monoclonal antibody (67852-1-Ig)

    WB analysis of HepG2 using 67852-1-Ig (same clone as 67852-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67852-1-Ig (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67852-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HuH-7 cells using AFP Monoclonal antibody (67852-1-Ig)

    WB analysis of HuH-7 using 67852-1-Ig (same clone as 67852-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 67852-1-Ig (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67852-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using AFP Monoclonal antibody (67852-1-Ig)

    WB analysis of human testis using 67852-1-Ig (same clone as 67852-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 67852-1-Ig (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67852-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using AFP Monoclonal antibody (67852-1-Ig)

    WB analysis of human placenta using 67852-1-Ig (same clone as 67852-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67852-1-Ig (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67852-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AFP Monoclonal antibody (67852-1-Ig)

    IHC staining of human liver cancer using 67852-1-Ig (same clone as 67852-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67852-1-Ig (AFP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67852-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using AFP Monoclonal antibody (67852-1-Ig)

    IHC staining of human liver using 67852-1-Ig (same clone as 67852-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67852-1-Ig (AFP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67852-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67852-1-PBS targets AFP in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AFP fusion protein Ag6089 相同性解析による交差性が予測される生物種
Full Name alpha-fetoprotein
Calculated molecular weight 69 kDa
Observed molecular weight 68-72 kDa
GenBank accession numberBC027881
Gene symbol AFP
Gene ID (NCBI) 174
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

AFP, also known as Alpha-fetoprotein, is a major plasma protein in the fetus and its concentration is very low in the adult (PubMed:24120489). AFP is considered a marker of hepatocyte precursors. AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma. High AFP concentrations have been correlated with tumor cell growth, indicating that AFP can serve as a tumor marker (PubMed: 18669658).