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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using AFP Recombinant antibody (81083-1-RR)

    WB analysis of HepG2 using 81083-1-RR (same clone as 81083-1-PBS)

    WB result of AFP antibody (81083-1-RR; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AFP transfected HepG2 cells. This data was developed using the same antibody clone with 81083-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AFP Recombinant antibody (81083-1-RR)

    WB analysis using 81083-1-RR (same clone as 81083-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 81083-1-RR (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81083-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AFP Recombinant antibody (81083-1-RR)

    WB analysis using 81083-1-RR (same clone as 81083-1-PBS)

    human placenta were subjected to SDS PAGE followed by western blot with 81083-1-RR (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81083-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HuH-7 cells using AFP Recombinant antibody (81083-1-RR)

    WB analysis of HuH-7 using 81083-1-RR (same clone as 81083-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 81083-1-RR (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81083-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using AFP Recombinant antibody (81083-1-RR)

    IHC staining of human colon cancer using 81083-1-RR (same clone as 81083-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81083-1-RR (AFP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81083-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using AFP Recombinant antibody (81083-1-RR)

    IHC staining of human colon cancer using 81083-1-RR (same clone as 81083-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81083-1-RR (AFP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81083-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81083-1-RR

    Affinity and Kinetic Characterization of 81083-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81083-1-RR against Human AFP were performed. The affinity constant is below 1 pM.

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  • Featured Product
  • KD/KO Validated

AFP Recombinant antibody, PBS Only

AFP Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 81083-1-PBS
Clone No.2D17

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, alpha fetoprotein, Alpha fetoglobulin

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81083-1-PBS targets AFP in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AFP fusion protein Ag6089 相同性解析による交差性が予測される生物種
Full Name alpha-fetoprotein
Calculated molecular weight 69 kDa
Observed molecular weight68-72 kDa
GenBank accession numberBC027881
Gene Symbol AFP
Gene ID (NCBI) 174
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02771
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

AFP, also known as Alpha-fetoprotein, is a major plasma protein in the fetus and its concentration is very low in the adult (PubMed:24120489). AFP is considered a marker of hepatocyte precursors. AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma. High AFP concentrations have been correlated with tumor cell growth, indicating that AFP can serve as a tumor marker (PubMed: 18669658).