AFP Recombinant antibody, PBS Only

WB result of AFP antibody (81083-1-RR; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AFP transfected HepG2 cells. This data was developed using the same antibody clone with 81083-1-PBS in a different storage buffer formulation.

HepG2 cells were subjected to SDS PAGE followed by western blot with 81083-1-RR (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81083-1-PBS in a different storage buffer formulation.

human placenta were subjected to SDS PAGE followed by western blot with 81083-1-RR (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81083-1-PBS in a different storage buffer formulation.

HuH-7 cells were subjected to SDS PAGE followed by western blot with 81083-1-RR (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81083-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81083-1-RR (AFP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81083-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81083-1-RR (AFP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81083-1-PBS in a different storage buffer formulation.

Biolayer interferometry (BLl) kinetic assays of 81083-1-RR against Human AFP were performed. The affinity constant is below 1 pM.