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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using AFP Recombinant antibody (81083-1-RR)

    WB analysis of HepG2 using 81083-1-RR (same clone as 81083-1-PBS)

    WB result of AFP antibody (81083-1-RR; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AFP transfected HepG2 cells. This data was developed using the same antibody clone with 81083-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AFP Recombinant antibody (81083-1-RR)

    WB analysis using 81083-1-RR (same clone as 81083-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 81083-1-RR (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81083-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AFP Recombinant antibody (81083-1-RR)

    WB analysis using 81083-1-RR (same clone as 81083-1-PBS)

    human placenta were subjected to SDS PAGE followed by western blot with 81083-1-RR (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81083-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HuH-7 cells using AFP Recombinant antibody (81083-1-RR)

    WB analysis of HuH-7 using 81083-1-RR (same clone as 81083-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 81083-1-RR (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81083-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AFP Recombinant antibody (81083-1-RR)

    IHC staining of human liver cancer using 81083-1-RR (same clone as 81083-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81083-1-RR (AFP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81083-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AFP Recombinant antibody (81083-1-RR)

    IHC staining of human liver cancer using 81083-1-RR (same clone as 81083-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81083-1-RR (AFP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81083-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using AFP Recombinant antibody (81083-1-RR)

    IHC staining of human colon cancer using 81083-1-RR (same clone as 81083-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81083-1-RR (AFP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81083-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using AFP Recombinant antibody (81083-1-RR)

    IHC staining of human colon cancer using 81083-1-RR (same clone as 81083-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81083-1-RR (AFP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81083-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81083-1-RR

    Affinity and Kinetic Characterization of 81083-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81083-1-RR against Human AFP were performed. The affinity constant is below 1 pM.

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  • Featured Product
  • KD/KO Validated

AFP Recombinant antibody, PBS Only

AFP Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 81083-1-PBS
Clone No.2D17

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

2D17, Alpha fetoglobulin, alpha fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81083-1-PBS targets AFP in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AFP fusion protein Ag6089 相同性解析による交差性が予測される生物種
Full Name alpha-fetoprotein
Calculated molecular weight 69 kDa
Observed molecular weight68-72 kDa
GenBank accession numberBC027881
Gene Symbol AFP
Gene ID (NCBI) 174
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02771
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

AFP (Alpha-fetoprotein) is a major plasma protein in the fetus and its concentration is very low in the adult (PMID:24120489). AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma, indicating that AFP can serve as a tumor marker (PMID: 18669658). AFP is also a glycosylated protein and based on its binding capability to lectin Lens Culinaris Agglutinin (LCA), and total AFP can be separated into three different glycoforms, AFP-L1, AFP-L2, and AFP-L3. Core-fucosylated form of AFP (AFP-L3) is a more specific indicator than total AFP for HCC (PMID: 33128033, 35458505)