- Featured Product
- KD/KO Validated
AFP Recombinant antibody
AFP Recombinant Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
2D17
Cat no : 81083-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, HuH-7 cells, human placenta tissue |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
81083-1-RR targets AFP in WB, IHC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | AFP fusion protein Ag6089 相同性解析による交差性が予測される生物種 |
Full Name | alpha-fetoprotein |
Calculated molecular weight | 69 kDa |
Observed molecular weight | 68-72 kDa |
GenBank accession number | BC027881 |
Gene symbol | AFP |
Gene ID (NCBI) | 174 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AFP (Alpha-fetoprotein) is a major plasma protein in the fetus and its concentration is very low in the adult (PMID:24120489). AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma, indicating that AFP can serve as a tumor marker (PMID: 18669658). AFP is also a glycosylated protein and based on its binding capability to lectin Lens Culinaris Agglutinin (LCA), and total AFP can be separated into three different glycoforms, AFP-L1, AFP-L2, and AFP-L3. Core-fucosylated form of AFP (AFP-L3) is a more specific indicator than total AFP for HCC (PMID: 33128033, 35458505)
Protocols
Product Specific Protocols | |
---|---|
WB protocol for AFP antibody 81083-1-RR | Download protocol |
IHC protocol for AFP antibody 81083-1-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |