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  • Featured Product
  • KD/KO Validated

AFP Recombinant antibody

AFP Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D17

Cat no : 81083-1-RR

Synonyms

Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, alpha fetoprotein, Alpha fetoglobulin

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using AFP Recombinant antibody (81083-1-RR)

    WB analysis of HepG2 using 81083-1-RR

    WB result of AFP antibody (81083-1-RR; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AFP transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using AFP Recombinant antibody (81083-1-RR)

    WB analysis using 81083-1-RR

    human placenta were subjected to SDS PAGE followed by western blot with 81083-1-RR (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using AFP Recombinant antibody (81083-1-RR)

    WB analysis using 81083-1-RR

    HepG2 cells were subjected to SDS PAGE followed by western blot with 81083-1-RR (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HuH-7 cells using AFP Recombinant antibody (81083-1-RR)

    WB analysis of HuH-7 using 81083-1-RR

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 81083-1-RR (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using AFP Recombinant antibody (81083-1-RR)

    IHC staining of human colon cancer using 81083-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81083-1-RR (AFP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using AFP Recombinant antibody (81083-1-RR)

    IHC staining of human colon cancer using 81083-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81083-1-RR (AFP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 81083-1-RR

    Affinity and Kinetic Characterization of 81083-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81083-1-RR against Human AFP were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHepG2 cells, HuH-7 cells, human placenta tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81083-1-RR targets AFP in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AFP fusion protein Ag6089 相同性解析による交差性が予測される生物種
Full Name alpha-fetoprotein
Calculated molecular weight 69 kDa
Observed molecular weight68-72 kDa
GenBank accession numberBC027881
Gene symbol AFP
Gene ID (NCBI) 174
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AFP (Alpha-fetoprotein) is a major plasma protein in the fetus and its concentration is very low in the adult (PMID:24120489). AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma, indicating that AFP can serve as a tumor marker (PMID: 18669658). AFP is also a glycosylated protein and based on its binding capability to lectin Lens Culinaris Agglutinin (LCA), and total AFP can be separated into three different glycoforms, AFP-L1, AFP-L2, and AFP-L3. Core-fucosylated form of AFP (AFP-L3) is a more specific indicator than total AFP for HCC (PMID: 33128033, 35458505)

Protocols

Product Specific Protocols
WB protocol for AFP antibody 81083-1-RRDownload protocol
IHC protocol for AFP antibody 81083-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols