AGER/RAGE Monoclonal antibody
AGER/RAGE Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, rat, pig and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
3D4G11
Cat no : 66833-1-Ig
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | pig lung tissue, rat lung tissue |
| Positive IHC detected in | human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
Product Information
66833-1-Ig targets AGER/RAGE in WB, IHC, ELISA applications and shows reactivity with human, rat, pig samples.
| Tested Reactivity | human, rat, pig |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | AGER/RAGE fusion protein Ag9657 相同性解析による交差性が予測される生物種 |
| Full Name | advanced glycosylation end product-specific receptor |
| Calculated molecular weight | 404 aa, 43 kDa |
| Observed molecular weight | 45 kDa |
| GenBank accession number | BC020669 |
| Gene symbol | AGER |
| Gene ID (NCBI) | 177 |
| RRID | AB_2882176 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Advanced glycosylation end product-specific receptor (AGER, also known as RAGE) is a multiligand receptor of the immunoglobulin superfamily. It interacts with distinct molecules implicated in homeostasis, development, and inflammation, and certain diseases such as diabetes and Alzheimer disease.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AGER/RAGE antibody 66833-1-Ig | Download protocol |
| IHC protocol for AGER/RAGE antibody 66833-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Signal Dapagliflozin alleviates advanced glycation end product induced podocyte injury through AMPK/mTOR mediated autophagy pathway | ||
Mol Immunol S100A11 regulates nasal epithelial cell remodeling and inflammation in CRSwNPs via the RAGE-mediated AMPK-STAT3 pathway. | ||
J Ethnopharmacol Zhongfeng Decoction attenuates cerebral ischemia-reperfusion injury by inhibiting autophagy via regulating the AGE-RAGE signaling pathway |
