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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AGER/RAGE Monoclonal antibody, PBS Only

AGER/RAGE Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3D4G11

Cat no : 66833-1-PBS

Synonyms

RAGE, AGER, Advanced glycosylation end product-specific receptor, 3D4G11

Validation Data Gallery

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  • Western Blot (WB) analysis of pig lung tissue using AGER/RAGE Monoclonal antibody (66833-1-Ig)

    WB analysis of pig lung using 66833-1-Ig (same clone as 66833-1-PBS)

    pig lung tissue were subjected to SDS PAGE followed by western blot with 66833-1-Ig (AGER antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66833-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat lung tissue using AGER/RAGE Monoclonal antibody (66833-1-Ig)

    WB analysis of rat lung using 66833-1-Ig (same clone as 66833-1-PBS)

    rat lung tissue were subjected to SDS PAGE followed by western blot with 66833-1-Ig (AGER antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66833-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using AGER/RAGE Monoclonal antibody (66833-1-Ig)

    IHC staining of human lung using 66833-1-Ig (same clone as 66833-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66833-1-Ig (AGER antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66833-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using AGER/RAGE Monoclonal antibody (66833-1-Ig)

    IHC staining of human lung using 66833-1-Ig (same clone as 66833-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66833-1-Ig (AGER antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66833-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66833-1-PBS targets AGER/RAGE in WB, IHC, ELISA applications and shows reactivity with human, rat, pig samples.

Tested Reactivity human, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AGER/RAGE fusion protein Ag9657 相同性解析による交差性が予測される生物種
Full Name advanced glycosylation end product-specific receptor
Calculated molecular weight 404 aa, 43 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC020669
Gene symbol AGER
Gene ID (NCBI) 177
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Advanced glycosylation end product-specific receptor (AGER, also known as RAGE) is a multiligand receptor of the immunoglobulin superfamily. It interacts with distinct molecules implicated in homeostasis, development, and inflammation, and certain diseases such as diabetes and Alzheimer disease.