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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AGT Monoclonal antibody

AGT Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B2F5

Cat no : 68020-1-Ig

Synonyms

AGT, Ang I Angiotensin 2, Ang II Angiotensin 3, Ang III, Angiotensin I, Angiotensin II, Angiotensin III, Angiotensinogen, ANHU, Des Asp[1] angiotensin II, FLJ92595, FLJ97926, Serpin A8, SERPINA8

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using AGT Monoclonal antibody (68020-1-Ig)

    WB analysis of human plasma using 68020-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 68020-1-Ig (AGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using AGT Monoclonal antibody (68020-1-Ig)

    WB analysis of human placenta using 68020-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68020-1-Ig (AGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig plasma using AGT Monoclonal antibody (68020-1-Ig)

    WB analysis of pig plasma using 68020-1-Ig

    pig plasma were subjected to SDS PAGE followed by western blot with 68020-1-Ig (AGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HuH-7 cells using AGT Monoclonal antibody (68020-1-Ig)

    WB analysis of HuH-7 using 68020-1-Ig

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 68020-1-Ig (AGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using AGT Monoclonal antibody (68020-1-Ig)

    WB analysis of THP-1 using 68020-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 68020-1-Ig (AGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using AGT Monoclonal antibody (68020-1-Ig)

    IHC staining of human kidney using 68020-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68020-1-Ig (AGT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using AGT Monoclonal antibody (68020-1-Ig)

    IHC staining of human kidney using 68020-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68020-1-Ig (AGT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using AGT Monoclonal antibody (68020-1-Ig)

    IHC staining of human liver using 68020-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68020-1-Ig (AGT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using AGT Monoclonal antibody (68020-1-Ig)

    IHC staining of human liver using 68020-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68020-1-Ig (AGT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using AGT Monoclonal antibody (68020-1-Ig)

    IF Staining of HepG2 using 68020-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AGT antibody (68020-1-Ig, Clone: 1B2F5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inhuman plasma, HuH-7 cells, THP-1 cells, human placenta tissue, pig plasma
Positive IHC detected inhuman kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68020-1-Ig targets AGT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, pig samples.

Tested Reactivity Human, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen AGT fusion protein Ag16658 相同性解析による交差性が予測される生物種
Full Name angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
Calculated molecular weight 485 aa, 53 kDa
Observed molecular weight52 kDa
GenBank accession numberBC011519
Gene symbol AGT
Gene ID (NCBI) 183
RRIDAB_2918765
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Angiotensinogen is a precursor of angiotensin II (Ang II), is expressed and synthesized largely in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. It has a key role in mediating vascular constriction and regulating salt and fluid homeostasis. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene also have been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.

Protocols

Product Specific Protocols
WB protocol for AGT antibody 68020-1-IgDownload protocol
IHC protocol for AGT antibody 68020-1-IgDownload protocol
IF protocol for AGT antibody 68020-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols