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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated AHCY Monoclonal antibody

AHCY Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F9B10

Cat no : CL488-66019

Synonyms

Adenosylhomocysteinase, AdoHcyase, AHCY, SAHH

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated AHCY Monoclonal anti (CL488-66019)

    IF Staining of HeLa using CL488-66019

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 AHCY antibody (CL488-66019, Clone: 1F9B10 ) at dilution of 1:1000.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66019 targets AHCY in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen AHCY fusion protein Ag18036 相同性解析による交差性が予測される生物種
Full Name S-adenosylhomocysteine hydrolase
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC011606
Gene symbol AHCY
Gene ID (NCBI) 191
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AHCY, also named as SAHH, belongs to the adenosylhomocysteinase family. AHCY is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore AHCY may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. AHCY catalyzes the reversible hydrolysis of S-adenosylhomocysteine to adenosine and L-homocysteine.(PMID:21220475).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 AHCY antibody CL488-66019Download protocol
Standard Protocols
Click here to view our Standard Protocols