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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated AHR Polyclonal antibody

AHR Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-28727

Synonyms

AHR, bHLHe76, Class E basic helix-loop-helix protein 76, Ah receptor

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 488-conjugated AHR Polyclonal antib (CL488-28727)

    IF Staining of A431 using CL488-28727

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 AHR antibody (CL488-28727) at dilution of 1:100.

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Tested Applications

Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-28727 targets AHR in IF/ICC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AHR fusion protein Ag28935 相同性解析による交差性が予測される生物種
Full Name aryl hydrocarbon receptor
Calculated molecular weight 848 aa, 96 kDa
Observed molecular weight105-110 kDa
GenBank accession numberBC070080
Gene symbol AHR
Gene ID (NCBI) 196
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism [PMID:18483242]. It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH)17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling [PMID:20423157,18540824]. It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 AHR antibody CL488-28727Download protocol
Standard Protocols
Click here to view our Standard Protocols