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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Fetuin-A/AHSG Monoclonal antibody

Fetuin-A/AHSG Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

1F6B9

Cat no : 66094-1-Ig

Synonyms

AHSG, Fetuin-A, Alpha 2 Z globulin, alpha 2 HS glycoprotein, AHS

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using Fetuin-A Monoclonal antibody (66094-1-Ig)

    WB analysis of HepG2 using 66094-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66094-1-Ig (Fetuin-A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human plasma using Fetuin-A Monoclonal antibody (66094-1-Ig)

    WB analysis of human plasma using 66094-1-Ig

    human blood were subjected to SDS PAGE followed by western blot with 66094-1-Ig (Fetuin-A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using Fetuin-A Monoclonal antibody (66094-1-Ig)

    WB analysis of human heart using 66094-1-Ig

    human heart tissue were subjected to SDS PAGE followed by western blot with 66094-1-Ig (Fetuin-A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using Fetuin-A Monoclonal antibody (66094-1-Ig)

    IP experiment of HepG2 using 66094-1-Ig

    IP result of anti-Fetuin-A (IP:66094-1-Ig, 3ug; Detection:66094-1-Ig 1:3000) with HepG2 cells lysate 6000ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Fetuin-A Monoclonal antibody (66094-1-Ig)

    IHC staining of human liver cancer using 66094-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 66094-1-Ig(Fetuin-A antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Fetuin-A Monoclonal antibody (66094-1-Ig)

    IHC staining of human liver cancer using 66094-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 66094-1-Ig(Fetuin-A antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Fetuin-A Monoclonal antibody (66094-1-Ig)

    IHC staining of human liver using 66094-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver using 66094-1-Ig(Fetuin-A antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Fetuin-A Monoclonal antibody (66094-1-Ig)

    IHC staining of human liver using 66094-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver using 66094-1-Ig(Fetuin-A antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Fetuin-A Monoclonal antibody (66094-1-Ig)

    IF Staining of HepG2 using 66094-1-Ig

    Immunofluorescent analysis of HepG2 cells, using AHSG antibody 66094-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

  • Flow cytometry (FC) experiment of HepG2 cells using Fetuin-A/AHSG Monoclonal antibody (66094-1-Ig)

    FC experiment of HepG2 using 66094-1-Ig

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Fetuin-A/AHSG Monoclonal antibody (66094-1-Ig, Clone:1F6B9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHepg2 cells, human heart tissue, human plasma tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66094-1-Ig targets Fetuin-A/AHSG in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Fetuin-A/AHSG fusion protein Ag10073 相同性解析による交差性が予測される生物種
Full Name alpha-2-HS-glycoprotein
Calculated molecular weight 367 aa, 39 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC048198
Gene symbol Fetuin-A
Gene ID (NCBI) 197
RRIDAB_11232222
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fetuin-A (alpha-2-HS-glycoprotein, AHSG), a liver borne plasma protein, contributes to the prevention of soft tissue calcification, modulates inflammation, reduces INS sensitivity and fosters weight gain following high fat diet or ageing. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues.

Protocols

Product Specific Protocols
WB protocol for Fetuin-A/AHSG antibody 66094-1-IgDownload protocol
IHC protocol for Fetuin-A/AHSG antibody 66094-1-IgDownload protocol
IF protocol for Fetuin-A/AHSG antibody 66094-1-IgDownload protocol
IP protocol for Fetuin-A/AHSG antibody 66094-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols