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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Fetuin-A/AHSG Monoclonal antibody, PBS Only

Fetuin-A/AHSG Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F6B9

Cat no : 66094-1-PBS

Synonyms

AHSG, Fetuin-A, Alpha 2 Z globulin, alpha 2 HS glycoprotein, AHS

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using Fetuin-A/AHSG Monoclonal antibody (66094-1-Ig)

    WB analysis of HepG2 using 66094-1-Ig (same clone as 66094-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66094-1-Ig (Fetuin-A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66094-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using Fetuin-A/AHSG Monoclonal antibody (66094-1-Ig)

    WB analysis of human heart using 66094-1-Ig (same clone as 66094-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66094-1-Ig (Fetuin-A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66094-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human plasma using Fetuin-A/AHSG Monoclonal antibody (66094-1-Ig)

    WB analysis of human plasma using 66094-1-Ig (same clone as 66094-1-PBS)

    human blood were subjected to SDS PAGE followed by western blot with 66094-1-Ig (Fetuin-A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66094-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using Fetuin-A/AHSG Monoclonal antibody (66094-1-Ig)

    IP experiment of HepG2 using 66094-1-Ig (same clone as 66094-1-PBS)

    IP result of anti-Fetuin-A (IP:66094-1-Ig, 3ug; Detection:66094-1-Ig 1:3000) with HepG2 cells lysate 6000ug. This data was developed using the same antibody clone with 66094-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Fetuin-A/AHSG Monoclonal antibody (66094-1-Ig)

    IHC staining of human liver cancer using 66094-1-Ig (same clone as 66094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 66094-1-Ig(Fetuin-A antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66094-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Fetuin-A/AHSG Monoclonal antibody (66094-1-Ig)

    IHC staining of human liver cancer using 66094-1-Ig (same clone as 66094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 66094-1-Ig(Fetuin-A antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66094-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Fetuin-A/AHSG Monoclonal antibody (66094-1-Ig)

    IHC staining of human liver using 66094-1-Ig (same clone as 66094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 66094-1-Ig(Fetuin-A antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66094-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Fetuin-A/AHSG Monoclonal antibody (66094-1-Ig)

    IHC staining of human liver using 66094-1-Ig (same clone as 66094-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 66094-1-Ig(Fetuin-A antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66094-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Fetuin-A/AHSG Monoclonal antibody (66094-1-Ig)

    IF Staining of HepG2 using 66094-1-Ig (same clone as 66094-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using AHSG antibody 66094-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 66094-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using Fetuin-A/AHSG Monoclonal antibody (66094-1-Ig)

    FC experiment of HepG2 using 66094-1-Ig (same clone as 66094-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Fetuin-A/AHSG Monoclonal antibody (66094-1-Ig, Clone:1F6B9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66094-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66094-1-PBS targets Fetuin-A/AHSG in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Fetuin-A/AHSG fusion protein Ag10073 相同性解析による交差性が予測される生物種
Full Name alpha-2-HS-glycoprotein
Calculated molecular weight 367 aa, 39 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC048198
Gene symbol Fetuin-A
Gene ID (NCBI) 197
RRIDAB_11232222
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Fetuin-A (alpha-2-HS-glycoprotein, AHSG), a liver borne plasma protein, contributes to the prevention of soft tissue calcification, modulates inflammation, reduces INS sensitivity and fosters weight gain following high fat diet or ageing. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues.