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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AIDA Monoclonal antibody (68153-1-Ig)

    WB analysis using 68153-1-Ig (same clone as 68153-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68153-1-Ig (AIDA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68153-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AIDA Monoclonal antibody (68153-1-Ig)

    WB analysis using 68153-1-Ig (same clone as 68153-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68153-1-Ig (AIDA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68153-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using AIDA Monoclonal antibody (68153-1-Ig)

    WB analysis of HepG2 using 68153-1-Ig (same clone as 68153-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68153-1-Ig (AIDA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68153-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using AIDA Monoclonal antibody (68153-1-Ig)

    IF Staining of A431 using 68153-1-Ig (same clone as 68153-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using AIDA antibody (68153-1-Ig, Clone: 1D11A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68153-1-PBS in a different storage buffer formulation.

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AIDA Monoclonal antibody, PBS Only

AIDA Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68153-1-PBS
Clone No.1D11A1

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

AIDA, C1orf80, FLJ12806, FLJ32421, RP11 378J18.7

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68153-1-PBS targets AIDA in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AIDA fusion protein Ag20597 相同性解析による交差性が予測される生物種
Full Name axin interactor, dorsalization associated
Calculated molecular weight 306 aa, 35 kDa
Observed molecular weight35 kDa
GenBank accession numberBC015535
Gene Symbol AIDA
Gene ID (NCBI) 64853
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96BJ3
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

AIDA, also named as C1orf80, acts as a ventralizing factor during embryogenesis. It inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. It's highest expression in the heart and skeletal muscle. AIDA has three isoforms with MW 35 kDa, 16 kDa and 26 kDa.