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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AIDA Monoclonal antibody

AIDA Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D11A1

Cat no : 68153-1-Ig

Synonyms

AIDA, C1orf80, FLJ12806, FLJ32421, RP11 378J18.7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AIDA Monoclonal antibody (68153-1-Ig)

    WB analysis using 68153-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68153-1-Ig (AIDA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using AIDA Monoclonal antibody (68153-1-Ig)

    WB analysis using 68153-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68153-1-Ig (AIDA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using AIDA Monoclonal antibody (68153-1-Ig)

    WB analysis of HepG2 using 68153-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68153-1-Ig (AIDA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using AIDA Monoclonal antibody (68153-1-Ig)

    IF Staining of A431 using 68153-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using AIDA antibody (68153-1-Ig, Clone: 1D11A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, COLO 320 cells, HepG2 cells, HT-29 cells, U2OS cells, K-562 cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68153-1-Ig targets AIDA in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AIDA fusion protein Ag20597 相同性解析による交差性が予測される生物種
Full Name axin interactor, dorsalization associated
Calculated molecular weight 306 aa, 35 kDa
Observed molecular weight35 kDa
GenBank accession numberBC015535
Gene symbol AIDA
Gene ID (NCBI) 64853
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AIDA, also named as C1orf80, acts as a ventralizing factor during embryogenesis. It inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. It's highest expression in the heart and skeletal muscle. AIDA has three isoforms with MW 35 kDa, 16 kDa and 26 kDa.

Protocols

Product Specific Protocols
WB protocol for AIDA antibody 68153-1-IgDownload protocol
IF protocol for AIDA antibody 68153-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols