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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated AIDA Monoclonal antibody

AIDA Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D11A1

Cat no : CL488-68153

Synonyms

AIDA, C1orf80, FLJ12806, FLJ32421, RP11 378J18.7

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 488-conjugated AIDA Monoclonal anti (CL488-68153)

    IF Staining of A431 using CL488-68153

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 AIDA antibody (CL488-68153, Clone: 1D11A1 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68153 targets AIDA in IF/ICC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AIDA fusion protein Ag20597 相同性解析による交差性が予測される生物種
Full Name axin interactor, dorsalization associated
Calculated molecular weight 306 aa, 35 kDa
Observed molecular weight35 kDa
GenBank accession numberBC015535
Gene symbol AIDA
Gene ID (NCBI) 64853
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AIDA, also named as C1orf80, acts as a ventralizing factor during embryogenesis. It inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. It's highest expression in the heart and skeletal muscle. AIDA has three isoforms with MW 35 kDa, 16 kDa and 26 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 AIDA antibody CL488-68153Download protocol
Standard Protocols
Click here to view our Standard Protocols