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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AIFM2/ FSP1 Monoclonal antibody

AIFM2/ FSP1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

1A2B2

Cat no : 68049-1-Ig

Synonyms

AMID, AIFM2, FSP1, Apoptosis-inducing factor homologous mitochondrion-associated inducer of death, Apoptosis inducing factor 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AIFM2/ FSP1 Monoclonal antibody (68049-1-Ig)

    WB analysis using 68049-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68049-1-Ig (AIFM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using AIFM2/ FSP1 Monoclonal antibody (68049-1-Ig)

    WB analysis of K-562 using 68049-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 68049-1-Ig (AIFM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NCI-H1299 cells using AIFM2/ FSP1 Monoclonal antibody (68049-1-Ig)

    WB analysis of NCI-H1299 using 68049-1-Ig

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 68049-1-Ig (AIFM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using AIFM2/ FSP1 Monoclonal antibody (68049-1-Ig)

    WB analysis of A549 using 68049-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 68049-1-Ig (AIFM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using AIFM2/ FSP1 Monoclonal antibody (68049-1-Ig)

    WB analysis of HEK-293 using 68049-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 68049-1-Ig (AIFM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using AIFM2/ FSP1 Monoclonal antibody (68049-1-Ig)

    WB analysis of MCF-7 using 68049-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 68049-1-Ig (AIFM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using AIFM2/ FSP1 Monoclonal antibody (68049-1-Ig)

    IP experiment of K-562 using 68049-1-Ig

    IP result of anti-AIFM2/ FSP1 (IP:68049-1-Ig, 4ug; Detection:68049-1-Ig 1:500) with K-562 cells lysate 1760 ug.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using AIFM2/ FSP1 Monoclonal antibody (68049-1-Ig)

    IHC staining of mouse liver using 68049-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68049-1-Ig (AIFM2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using AIFM2/ FSP1 Monoclonal antibody (68049-1-Ig)

    IHC staining of mouse liver using 68049-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68049-1-Ig (AIFM2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of H9C2 cells using AIFM2/ FSP1 Monoclonal antibody (68049-1-Ig)

    IF Staining of H9C2 using 68049-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using AIFM2/ FSP1 antibody (68049-1-Ig, Clone: 1A2B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of K-562 cells using AIFM2/ FSP1 Monoclonal antibody (68049-1-Ig)

    FC experiment of K-562 using 68049-1-Ig

    1X10^6 K-562 cells were intracellularly stained with 0.5 ug Anti-Human AIFM2 (68049-1-Ig, Clone:1A2B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHepG2 cells, NCI-H1299 cells, A549 cells, HEK-293 cells, MCF-7 cells, K-562 cells, L02 cells
Positive IP detected inK-562 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inH9C2 cells
Positive FC (Intra) detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

68049-1-Ig targets AIFM2/ FSP1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AIFM2/ FSP1 fusion protein Ag30516 相同性解析による交差性が予測される生物種
Full Name apoptosis-inducing factor, mitochondrion-associated, 2
Calculated molecular weight 41 kDa
Observed molecular weight41 kDa
GenBank accession numberBC023601
Gene symbol AIFM2/ FSP1
Gene ID (NCBI) 84883
RRIDAB_2918791
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis Inducing Factor), a mitochondrion-associated enzyme which relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.

Protocols

Product Specific Protocols
WB protocol for AIFM2/ FSP1 antibody 68049-1-IgDownload protocol
IHC protocol for AIFM2/ FSP1 antibody 68049-1-IgDownload protocol
IF protocol for AIFM2/ FSP1 antibody 68049-1-IgDownload protocol
IP protocol for AIFM2/ FSP1 antibody 68049-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Redox Biol

SENP3 sensitizes macrophages to ferroptosis via de-SUMOylation of FSP1

Authors - Xuelian Chen
View Article
humanWB

Free Radic Biol Med

TRPV3 Facilitates Lipolysis and Attenuates Diet-Induced Obesity via Activation of the NRF2/FSP1 Signaling Axis

Authors - Yongyan Hu
View Article