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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AIFM2/ FSP1 Monoclonal antibody, PBS Only

AIFM2/ FSP1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A2B2

Cat no : 68049-1-PBS

Synonyms

AMID, AIFM2, FSP1, Apoptosis-inducing factor homologous mitochondrion-associated inducer of death, Apoptosis inducing factor 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AIFM2/ FSP1 Monoclonal antibody (68049-1-Ig)

    WB analysis using 68049-1-Ig (same clone as 68049-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68049-1-Ig (AIFM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using AIFM2/ FSP1 Monoclonal antibody (68049-1-Ig)

    WB analysis of K-562 using 68049-1-Ig (same clone as 68049-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 68049-1-Ig (AIFM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCI-H1299 cells using AIFM2/ FSP1 Monoclonal antibody (68049-1-Ig)

    WB analysis of NCI-H1299 using 68049-1-Ig (same clone as 68049-1-PBS)

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 68049-1-Ig (AIFM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using AIFM2/ FSP1 Monoclonal antibody (68049-1-Ig)

    WB analysis of A549 using 68049-1-Ig (same clone as 68049-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 68049-1-Ig (AIFM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using AIFM2/ FSP1 Monoclonal antibody (68049-1-Ig)

    WB analysis of HEK-293 using 68049-1-Ig (same clone as 68049-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 68049-1-Ig (AIFM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using AIFM2/ FSP1 Monoclonal antibody (68049-1-Ig)

    WB analysis of MCF-7 using 68049-1-Ig (same clone as 68049-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 68049-1-Ig (AIFM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using AIFM2/ FSP1 Monoclonal antibody (68049-1-Ig)

    IP experiment of K-562 using 68049-1-Ig (same clone as 68049-1-PBS)

    IP result of anti-AIFM2/ FSP1 (IP:68049-1-Ig, 4ug; Detection:68049-1-Ig 1:500) with K-562 cells lysate 1760 ug. This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using AIFM2/ FSP1 Monoclonal antibody (68049-1-Ig)

    IHC staining of mouse liver using 68049-1-Ig (same clone as 68049-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68049-1-Ig (AIFM2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using AIFM2/ FSP1 Monoclonal antibody (68049-1-Ig)

    IHC staining of mouse liver using 68049-1-Ig (same clone as 68049-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68049-1-Ig (AIFM2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of H9C2 cells using AIFM2/ FSP1 Monoclonal antibody (68049-1-Ig)

    IF Staining of H9C2 using 68049-1-Ig (same clone as 68049-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using AIFM2/ FSP1 antibody (68049-1-Ig, Clone: 1A2B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of K-562 cells using AIFM2/ FSP1 Monoclonal antibody (68049-1-Ig)

    FC experiment of K-562 using 68049-1-Ig (same clone as 68049-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.5 ug Anti-Human AIFM2 (68049-1-Ig, Clone:1A2B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68049-1-PBS targets AIFM2/ FSP1 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with mouse, human samples.

Tested Reactivity mouse, human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AIFM2/ FSP1 fusion protein Ag30516 相同性解析による交差性が予測される生物種
Full Name apoptosis-inducing factor, mitochondrion-associated, 2
Calculated molecular weight 41 kDa
Observed molecular weight41 kDa
GenBank accession numberBC023601
Gene symbol AIFM2/ FSP1
Gene ID (NCBI) 84883
RRIDAB_2918791
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis Inducing Factor), a mitochondrion-associated enzyme which relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.