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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AIFM2/ FSP1 Recombinant antibody, PBS Only

AIFM2/ FSP1 Recombinant Antibody for WB, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2J1

Cat no : 82974-1-PBS

Synonyms

AIFM2, AMID, Apoptosis inducing factor 2, p53 responsive gene 3 protein, PRG3, RP11 367H5.2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AIFM2/ FSP1 Recombinant antibody (82974-1-RR)

    WB analysis using 82974-1-RR (same clone as 82974-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82974-1-RR (AIFM2/ FSP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82974-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using AIFM2/ FSP1 Recombinant antibody (82974-1-RR)

    IP experiment of HepG2 using 82974-1-RR (same clone as 82974-1-PBS)

    IP result of anti-AIFM2/ FSP1 (IP:82974-1-RR, 4ug; Detection:82974-1-RR 1:3000) with HepG2 cells lysate 1085 ug. This data was developed using the same antibody clone with 82974-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82974-1-PBS targets AIFM2/ FSP1 in WB, IP, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AIFM2/ FSP1 fusion protein Ag13412 相同性解析による交差性が予測される生物種
Full Name apoptosis-inducing factor, mitochondrion-associated, 2
Calculated molecular weight 41 kDa
Observed molecular weight41 kDa
GenBank accession numberBC023601
Gene symbol AIFM2
Gene ID (NCBI) 84883
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis Inducing Factor), a mitochondrion-associated enzyme which relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.