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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AIFM2/ FSP1 Recombinant antibody, PBS Only

AIFM2/ FSP1 Recombinant Antibody for WB, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2J1

Cat no : 82974-1-PBS

Synonyms

AMID, AIFM2, FSP1, Apoptosis-inducing factor homologous mitochondrion-associated inducer of death, Apoptosis inducing factor 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AIFM2/ FSP1 Recombinant antibody (82974-1-RR)

    WB analysis using 82974-1-RR (same clone as 82974-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82974-1-RR (AIFM2/ FSP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82974-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using AIFM2/ FSP1 Recombinant antibody (82974-1-RR)

    IP experiment of HepG2 using 82974-1-RR (same clone as 82974-1-PBS)

    IP result of anti-AIFM2/ FSP1 (IP:82974-1-RR, 4ug; Detection:82974-1-RR 1:3000) with HepG2 cells lysate 1085 ug. This data was developed using the same antibody clone with 82974-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using AIFM2/ FSP1 Recombinant antibody (82974-1-RR)

    FC experiment of A549 using 82974-1-RR (same clone as 82974-1-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug AIFM2/ FSP1 Recombinant antibody (82974-1-RR, Clone:2J1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82974-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82974-1-RR

    Affinity and Kinetic Characterization of 82974-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82974-1-RR against Human AIFM2/ FSP1 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82974-1-PBS targets AIFM2/ FSP1 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AIFM2/ FSP1 fusion protein Ag13412 相同性解析による交差性が予測される生物種
Full Name apoptosis-inducing factor, mitochondrion-associated, 2
Calculated molecular weight 41 kDa
Observed molecular weight41 kDa
GenBank accession numberBC023601
Gene symbol AIFM2/ FSP1
Gene ID (NCBI) 84883
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis Inducing Factor), a mitochondrion-associated enzyme which relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.