AIFM2/ FSP1 Recombinant antibody
AIFM2/ FSP1 Recombinant Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, ELISA
Conjugate
Unconjugated
CloneNo.
2J1
Cat no : 82974-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HepG2 cells |
Positive IP detected in | HepG2 cells |
Positive FC (Intra) detected in | A549 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
82974-1-RR targets AIFM2/ FSP1 in WB, IP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | AIFM2/ FSP1 fusion protein Ag13412 相同性解析による交差性が予測される生物種 |
Full Name | apoptosis-inducing factor, mitochondrion-associated, 2 |
Calculated molecular weight | 41 kDa |
Observed molecular weight | 41 kDa |
GenBank accession number | BC023601 |
Gene symbol | AIFM2/ FSP1 |
Gene ID (NCBI) | 84883 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis Inducing Factor), a mitochondrion-associated enzyme which relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.
Protocols
Product Specific Protocols | |
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WB protocol for AIFM2/ FSP1 antibody 82974-1-RR | Download protocol |
IP protocol for AIFM2/ FSP1 antibody 82974-1-RR | Download protocol |
FC protocol for AIFM2/ FSP1 antibody 82974-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |