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CoraLite® Plus 488-conjugated AIM2 Monoclonal antibody
AIM2 Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, rabbit
Applications
WB
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3E12A8
Cat no : CL488-66902
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-66902 targets AIM2 in WB applications and shows reactivity with human, mouse, rat, rabbit samples.
| Tested Reactivity | human, mouse, rat, rabbit |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | AIM2 fusion protein Ag14607 相同性解析による交差性が予測される生物種 |
| Full Name | absent in melanoma 2 |
| Calculated molecular weight | 343 aa, 39 kDa |
| Observed molecular weight | 37-39 and 53 kDa |
| GenBank accession number | BC010940 |
| Gene symbol | AIM2 |
| Gene ID (NCBI) | 9447 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID: 22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831]. 66902-1-Ig antibody detects the 37-39 and 53 kDa protein in SDS-PAGE similar to the paper published. [PMID: 28809949]
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 488 AIM2 antibody CL488-66902 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
