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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AK2 Recombinant antibody (85406-3-RR)

    WB analysis using 85406-3-RR (same clone as 85406-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85406-3-RR (AK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85406-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01927-3

    Sandwich ELISA standard curve of MP01927-3

    Sandwich ELISA standard curve of MP01927-3, Human AK2 Recombinant Matched Antibody Pair - PBS only. 85406-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1464. 85406-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

  • Sample test of MP01927-3

    Sample test of MP01927-3

    The mean AK2 concentration was determined to be 6.31 ng/mL in HeLa cell extract based on a 2.10 mg/mL extract load and 5.60 ng/mL in HL-60 cell extract based on a 1.50 mg/mL extract load.

  • Cytometric bead array standard curve of MP01927-1

    Cytometric bead array standard curve of MP01927-1

    Cytometric bead array standard curve of MP01927-1, AK2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85406-3-PBS. Detection antibody: 85406-1-PBS. Standard: Ag1464. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 85406-3-RR

    Affinity and Kinetic Characterization of 85406-3-RR

    Biolayer interferometry (BLl) kinetic assays of 85406-3-RR against Human AK2 were performed. The affinity constant is 0.428 nM.

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AK2 Recombinant antibody, PBS Only (Capture)

AK2 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 85406-3-PBS
Clone No.242899H7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

242899H7, adenylate kinase 2, Adenylate kinase 2, mitochondrial, N-terminally processed, Adenylate monophosphate kinase, ADK2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85406-3-PBS targets AK2 as part of a matched antibody pair:

MP01927-1: 85406-3-PBS capture and 85406-1-PBS detection (validated in Cytometric bead array)

MP01927-3: 85406-3-PBS capture and 85406-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AK2 fusion protein Ag1464 相同性解析による交差性が予測される生物種
Full Name adenylate kinase 2
Calculated molecular weight 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC009405
Gene Symbol AK2
Gene ID (NCBI) 204
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP54819
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

AK2(Adenylate kinase 2, mitochondrial ) is also named as ADK2 and belongs to the adenylate kinase family. It plays a unique role in energy metabolism and energy transfer by regulating the ATP/ADP rate between the cytoplasma matrix and the mitochondria and it is increased expression during adipocyte and B cell differentiation(PMID:23020757). AK2 catalyzes the reversible transfer of a phosphoryl group from ATP to AMP(PMID:19043417). Defects in AK2 are the cause of reticular dysgenesis (RDYS)(PMID:19043416). It has 6 isoforms produced by alternative splicing.