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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AK2 Recombinant antibody (85406-3-RR)

    WB analysis using 85406-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85406-3-RR (AK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85406-3-RR

    Affinity and Kinetic Characterization of 85406-3-RR

    Biolayer interferometry (BLl) kinetic assays of 85406-3-RR against Human AK2 were performed. The affinity constant is 0.428 nM.

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AK2 Recombinant antibody

AK2 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85406-3-RR
Clone No.242899H7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

242899H7, adenylate kinase 2, Adenylate kinase 2, mitochondrial, N-terminally processed, Adenylate monophosphate kinase, ADK2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, HL-60 cells, Raji cells, mouse kidney tissue, rat kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85406-3-RR targets AK2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AK2 fusion protein Ag1464 相同性解析による交差性が予測される生物種
Full Name adenylate kinase 2
Calculated molecular weight 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC009405
Gene Symbol AK2
Gene ID (NCBI) 204
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP54819
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AK2(Adenylate kinase 2, mitochondrial ) is also named as ADK2 and belongs to the adenylate kinase family. It plays a unique role in energy metabolism and energy transfer by regulating the ATP/ADP rate between the cytoplasma matrix and the mitochondria and it is increased expression during adipocyte and B cell differentiation(PMID:23020757). AK2 catalyzes the reversible transfer of a phosphoryl group from ATP to AMP(PMID:19043417). Defects in AK2 are the cause of reticular dysgenesis (RDYS)(PMID:19043416). It has 6 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for AK2 antibody 85406-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols