Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | MCF7 cells, human liver tissue |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3600 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
13206-1-AP targets AK3L1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | AK3L1 fusion protein Ag3966 相同性解析による交差性が予測される生物種 |
| Full Name | adenylate kinase 3-like 1 |
| Calculated molecular weight | 223 aa, 25 kDa |
| Observed molecular weight | 30 kDa |
| GenBank accession number | BC040224 |
| Gene Symbol | AK3L1 |
| Gene ID (NCBI) | 205 |
| RRID | AB_2225456 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P27144 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AK3L1(Adenylate kinase 3-like) is also named as AK4, AK3L1 and belongs to the adenylate kinase family. It catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. It is important for maintenance of homeostasis of the adenine and guanine nucleotide pools. Expression is highest in kidney and heart, moderate in liver, weak in brain, and barely detectable in placenta and lung by northern blot(PMID:11485571). This antibody may also recognize AK3 due to the high homology.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AK3L1 antibody 13206-1-AP | Download protocol |
| IHC protocol for AK3L1 antibody 13206-1-AP | Download protocol |
| IF protocol for AK3L1 antibody 13206-1-AP | Download protocol |
| IP protocol for AK3L1 antibody 13206-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Cell Int The miR-199a-3p regulates the radioresistance of esophageal cancer cells via targeting the AK4 gene.
| ||
Exp Brain Res MicroRNA-124 conducts neuroprotective effect via inhibiting AK4/ATF3 after subarachnoid hemorrhage |