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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated AK3L1 Polyclonal antibody

AK3L1 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-13206

Synonyms

Adenylate kinase 3 like, adenylate kinase 3 like 1, AK3, AK3L1, AK4, ATP AMP transphosphorylase

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite® Plus 488-conjugated AK3L1 Polyclonal ant (CL488-13206)

    IF Staining of MCF-7 using CL488-13206

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 AK3L1 antibody (CL488-13206) at dilution of 1:200, CL594-Phalloidin (red).

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Tested Applications

Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-13206 targets AK3L1 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AK3L1 fusion protein Ag3966 相同性解析による交差性が予測される生物種
Full Name adenylate kinase 3-like 1
Calculated molecular weight 223 aa, 25 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC040224
Gene symbol AK3L1
Gene ID (NCBI) 205
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AK3L1(Adenylate kinase 3-like) is also named as AK4, AK3L1 and belongs to the adenylate kinase family. It catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. It is important for maintenance of homeostasis of the adenine and guanine nucleotide pools. Expression is highest in kidney and heart, moderate in liver, weak in brain, and barely detectable in placenta and lung by northern blot(PMID:11485571). This antibody may also recognize AK3 due to the high homology.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 AK3L1 antibody CL488-13206Download protocol
Standard Protocols
Click here to view our Standard Protocols