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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AKAP3 Monoclonal antibody

AKAP3 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3C11H9

Cat no : 66255-1-Ig

Synonyms

A kinase anchor protein 3, AKAP 110, AKAP 3, AKAP110, AKAP3, Cancer/testis antigen 82, CT82, Fibrousheathin 1, Fibrousheathin I, FSP95, PRKA3, SOB1, Sperm oocyte binding protein

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using AKAP3 Monoclonal antibody (66255-1-Ig)

    WB analysis of human testis using 66255-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 66255-1-Ig (AKAP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using AKAP3 Monoclonal antibody (66255-1-Ig)

    IHC staining of mouse testis using 66255-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66255-1-Ig (AKAP3 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using AKAP3 Monoclonal antibody (66255-1-Ig)

    IHC staining of mouse testis using 66255-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66255-1-Ig (AKAP3 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using AKAP3 Monoclonal antibody (66255-1-Ig)

    IF Staining of mouse testis using 66255-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using AKAP3 antibody (66255-1-Ig, Clone: 3C11H9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using AKAP3 Monoclonal antibody (66255-1-Ig)

    IF Staining of mouse testis using 66255-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using AKAP3 antibody (66255-1-Ig, Clone: 3C11H9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inhuman testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66255-1-Ig targets AKAP3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen AKAP3 fusion protein Ag4885 相同性解析による交差性が予測される生物種
Full Name A kinase (PRKA) anchor protein 3
Calculated molecular weight 95 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC047535
Gene symbol AKAP3
Gene ID (NCBI) 10566
RRIDAB_2881643
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AKAP3 is a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. AKAP3 is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. AKAP3 is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction.

Protocols

Product Specific Protocols
WB protocol for AKAP3 antibody 66255-1-IgDownload protocol
IHC protocol for AKAP3 antibody 66255-1-IgDownload protocol
IF protocol for AKAP3 antibody 66255-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols