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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AKAP3 Monoclonal antibody, PBS Only

AKAP3 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C11H9

Cat no : 66255-1-PBS

Synonyms

A kinase anchor protein 3, AKAP 110, AKAP 3, AKAP110, AKAP3, Cancer/testis antigen 82, CT82, Fibrousheathin 1, Fibrousheathin I, FSP95, PRKA3, SOB1, Sperm oocyte binding protein

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using AKAP3 Monoclonal antibody (66255-1-Ig)

    WB analysis of human testis using 66255-1-Ig (same clone as 66255-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 66255-1-Ig (AKAP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66255-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using AKAP3 Monoclonal antibody (66255-1-Ig)

    IHC staining of mouse testis using 66255-1-Ig (same clone as 66255-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66255-1-Ig (AKAP3 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66255-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using AKAP3 Monoclonal antibody (66255-1-Ig)

    IHC staining of mouse testis using 66255-1-Ig (same clone as 66255-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66255-1-Ig (AKAP3 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66255-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using AKAP3 Monoclonal antibody (66255-1-Ig)

    IF Staining of mouse testis using 66255-1-Ig (same clone as 66255-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using AKAP3 antibody (66255-1-Ig, Clone: 3C11H9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66255-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using AKAP3 Monoclonal antibody (66255-1-Ig)

    IF Staining of mouse testis using 66255-1-Ig (same clone as 66255-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using AKAP3 antibody (66255-1-Ig, Clone: 3C11H9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66255-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66255-1-PBS targets AKAP3 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen AKAP3 fusion protein Ag4885 相同性解析による交差性が予測される生物種
Full Name A kinase (PRKA) anchor protein 3
Calculated molecular weight 95 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC047535
Gene symbol AKAP3
Gene ID (NCBI) 10566
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

AKAP3 is a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. AKAP3 is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. AKAP3 is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction.