- Featured Product
- KD/KO Validated
CoraLite®488-conjugated AKT Monoclonal antibody
AKT Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB, IF/ICC
Conjugate
CoraLite®488 Fluorescent Dye
CloneNo.
2C5D1
Cat no : CL488-60203
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
CL488-60203 targets AKT in WB, IF/ICC applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | AKT fusion protein Ag16695 相同性解析による交差性が予測される生物種 |
| Full Name | v-akt murine thymoma viral oncogene homolog 1 |
| Calculated molecular weight | 56 kDa |
| GenBank accession number | BC000479 |
| Gene symbol | AKT1 |
| Gene ID (NCBI) | 207 |
| RRID | AB_2883119 |
| Conjugate | CoraLite®488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 491 nm / 516 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. This antibody detects all the members of AKT with/without phospho- modification. This antibody is CL488(Ex/Em 488 nm/515 nm) conjugated.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL488 AKT antibody CL488-60203 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Clin Invest Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling. | ||
Cell Death Discov MCM2 promotes the stemness and sorafenib resistance of hepatocellular carcinoma cells via hippo signaling |
