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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AKT2 Polyclonal antibody

AKT2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 28113-1-AP

Synonyms

PKBBETA, PKBB, PKB beta, EC:2.7.11.1, AKT2 protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AKT2 Polyclonal antibody (28113-1-AP)

    WB analysis using 28113-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28113-1-AP (AKT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using AKT2 Polyclonal antibody (28113-1-AP)

    IHC staining of human liver using 28113-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28113-1-AP (AKT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using AKT2 Polyclonal antibody (28113-1-AP)

    IHC staining of human liver using 28113-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28113-1-AP (AKT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using AKT2 Polyclonal antibody (28113-1-AP)

    IF Staining of NIH/3T3 using 28113-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using AKT2 antibody (28113-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of NIH/3T3 cells using AKT2 Polyclonal antibody (28113-1-AP)

    FC experiment of NIH/3T3 using 28113-1-AP

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Anti-Human AKT2 (28113-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inNIH/3T3 cells, mouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

28113-1-AP targets AKT2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKT2 fusion protein Ag27005 相同性解析による交差性が予測される生物種
Full Name v-akt murine thymoma viral oncogene homolog 2
Observed molecular weight56 kDa
GenBank accession numberBC120994
Gene symbol AKT2
Gene ID (NCBI) 208
RRIDAB_2881064
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis and their activation has been observed in a wide variety of cancers. AKT2 is mainly involved in cancer cell survival, apoptosis inhibition, migration and invasion(PMID:21979951). Defects in AKT2 are a cause of susceptibility to breast cancer (BC). AKT2 promotes metastasis of tumor cells without affecting the latency of tumor development. And defects in AKT2 are a cause of non-insulin-dependent diabetes mellitus (NIDDM) and hypoinsulinemic hypoglycemia with hemihypertrophy (HIHGHH). The full length protein has four glycosylation sites.

Protocols

Product Specific Protocols
WB protocol for AKT2 antibody 28113-1-APDownload protocol
IHC protocol for AKT2 antibody 28113-1-APDownload protocol
IF protocol for AKT2 antibody 28113-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Biochem Cell Biol

AKT serine/threonine kinase 2-mediated phosphorylation of fascin threonine 403 regulates esophageal cancer progression.

Authors - Zhi-Da Zhang
View Article
mouseWB

Chem Res Toxicol

Novel Dual PPAR δ/γ Partial Agonist Induces Hepatic Lipid Accumulation through Direct Binding and Inhibition of AKT1 Phosphorylation, Mediating CD36 Upregulation

Authors - Xiaotong Cai
View Article