CoraLite® Plus 488-conjugated AKT2 Polyclonal antibody
AKT2 Polyclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-28113
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | NIH/3T3 cells |
Positive FC (Intra) detected in | NIH/3T3 cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-28113 targets AKT2 in IF/ICC, FC (Intra) applications and shows reactivity with Human, mouse samples.
Tested Reactivity | Human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | AKT2 fusion protein Ag27005 相同性解析による交差性が予測される生物種 |
Full Name | v-akt murine thymoma viral oncogene homolog 2 |
Observed molecular weight | 56 kDa |
GenBank accession number | BC120994 |
Gene symbol | AKT2 |
Gene ID (NCBI) | 208 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis and their activation has been observed in a wide variety of cancers. AKT2 is mainly involved in cancer cell survival, apoptosis inhibition, migration and invasion(PMID:21979951). Defects in AKT2 are a cause of susceptibility to breast cancer (BC). AKT2 promotes metastasis of tumor cells without affecting the latency of tumor development. And defects in AKT2 are a cause of non-insulin-dependent diabetes mellitus (NIDDM) and hypoinsulinemic hypoglycemia with hemihypertrophy (HIHGHH). The full length protein has four glycosylation sites.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 AKT2 antibody CL488-28113 | Download protocol |
FC protocol for CL Plus 488 AKT2 antibody CL488-28113 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |