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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human ALCAM (3A6) Mouse IgG2a Recombinant Antibody, PBS Only

ALCAM Recombinant Antibody for IF/ICC, FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, FC

Conjugate

Unconjugated

CloneNo.

3A6

Cat no : 65611-1-PBS

Synonyms

MEMD, CD166, 3A6

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using Anti-Human ALCAM (3A6) Mouse IgG2a Recombinant Ant (65611-1-MR)

    IF Staining of hTERT-RPE1 using 65611-1-MR (same clone as 65611-1-PBS)

    Immunofluorescent analysis of un-fixed hTERT-RPE1 cells using ALCAM antibody (65611-1-MR, Clone: 3A6 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). This data was developed using the same antibody clone with 65611-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human ALCAM (3A6) Mouse IgG2a Recombinant Ant (65611-1-MR)

    FC experiment of human PBMCs using 65611-1-MR (same clone as 65611-1-PBS)

    1x10^6 PHA treated human PBMCs were surface stained with 0.25 ug Anti-Human ALCAM Mouse IgG2a Recombinant Antibody (65611-1-MR, Clone: 3A6) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Untreated human PBMCs were surface stained with 0.25 ug Anti-Human ALCAM Mouse IgG2a Recombinant Antibody (65611-1-MR, Clone: 3A6) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) (black). Cells were not fixed. This data was developed using the same antibody clone with 65611-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65611-1-PBS targets ALCAM in IF/ICC, FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Cultured human thymic epithelial cells 相同性解析による交差性が予測される生物種
Full Name activated leukocyte cell adhesion molecule
Calculated molecular weight 105 kDa
GenBank accession numberBC057809
Gene symbol ALCAM
Gene ID (NCBI) 214
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues and cell types, including activated T cells and monocytes, epithelial cells, fibroblasts, neuronal cells, hepatocytes, and bone marrow mesenchymal stem cells (PMID: 7760007; 25221999). Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).