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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Atlantic Blue™ Anti-Human ALCAM (3A6) (AB-65223)

    FC experiment of human PBMCs using AB-65223

    1X10^6 PHA activated human PBMCs were surface stained with 5 ul Atlantic Blue™ Anti-Human ALCAM (AB-65223, Clone:3A6). Cells were not fixed. Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Atlantic Blue™ Anti-Human ALCAM (3A6) (AB-65223)

    FC experiment of human PBMCs using AB-65223

    1X10^6 PHA activated human PBMCs were surface stained with 5 ul Atlantic Blue™ Anti-Human ALCAM (AB-65223, Clone:3A6). Cells were not fixed. Monocytes were gated.

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Atlantic Blue™ Anti-Human ALCAM (3A6)

ALCAM Monoclonal Antibody for FC
Cat No. AB-65223
Clone No.3A6

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Synonyms

ALCAM, CD166, CD166 antigen, FLJ38514, MEMD

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Atlantic Blue™
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC detected inPHA treated human PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

AB-65223 targets ALCAM in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Cultured human thymic epithelial cells 相同性解析による交差性が予測される生物種
Full Name activated leukocyte cell adhesion molecule
Calculated molecular weight 105 kDa
GenBank accession numberBC057809
Gene Symbol ALCAM
Gene ID (NCBI) 214
RRIDAB_2935200
Conjugate Atlantic Blue™ Fluorescent Dye
Excitation/Emission maxima wavelengths404 nm / 458 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDQ13740
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues and cell types, including activated T cells and monocytes, epithelial cells, fibroblasts, neuronal cells, hepatocytes, and bone marrow mesenchymal stem cells (PMID: 7760007; 25221999). Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).

Protocols

Product Specific Protocols
FC protocol for Atlantic Blue™ ALCAM antibody AB-65223Download protocol
Standard Protocols
Click here to view our Standard Protocols