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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CoraLite® Plus 647-conjugated ALCAM Monoclonal ant (CL647-67768)

    IF Staining of human breast cancer using CL647-67768

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 647 ALCAM antibody (CL647-67768, Clone: 1F7D6 ) at dilution of 1:200.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CoraLite® Plus 647-conjugated ALCAM Monoclonal antibody

ALCAM Monoclonal Antibody for IF-P
Cat No. CL647-67768
Clone No.1F7D6

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF-P

Synonyms

ALCAM, CD166, CD166 antigen, FLJ38514, MEMD

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inhuman breast cancer tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-67768 targets ALCAM in IF-P applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ALCAM fusion protein Ag16892 相同性解析による交差性が予測される生物種
Full Name activated leukocyte cell adhesion molecule
Calculated molecular weight 583 aa, 65 kDa
Observed molecular weight90-100 kDa
GenBank accession numberBC137097
Gene Symbol ALCAM
Gene ID (NCBI) 214
RRIDAB_2920309
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13740
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues. Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 ALCAM antibody CL647-67768Download protocol
Standard Protocols
Click here to view our Standard Protocols