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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated ALDH3A1 Monoclonal antibody

ALDH3A1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1D4G7

Cat no : CL594-68036

Synonyms

Aldehyde dehydrogenase 3, ALDH3, ALDH3A1, ALDHIII

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CoraLite®594-conjugated ALDH3A1 Monoclonal antibod (CL594-68036)

    IF Staining of HEK-293 using CL594-68036

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite®594 ALDH3A1 antibody (CL594-68036, Clone: 1D4G7 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HEK-293 cells using CoraLite®594-conjugated ALDH3A1 Monoclonal antibod (CL594-68036)

    FC experiment of HEK-293 using CL594-68036

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human ALDH3A1 (CL594-68036, Clone:1D4G7) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHEK-293 cells
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-68036 targets ALDH3A1 in IF/ICC, FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ALDH3A1 fusion protein Ag8125 相同性解析による交差性が予測される生物種
Full Name aldehyde dehydrogenase 3 family, memberA1
Calculated molecular weight 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC004370
Gene symbol ALDH3A1
Gene ID (NCBI) 218
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ALDH3A1(Aldehyde dehydrogenase, dimeric NADP-preferring), also named as ALDH3, is a member of the ALDH superfamily, which consists of NAD(P)+-dependent enzymes that catalyse the oxidation of a wide variety of endogenously produced and exogenous aldehydes to their corresponding acids. It is expressed at high concentrations in the mammalian cornea and it is believed that it protects this vital tissue and the rest of the eye against UVlight-induced damage.(PMID:12943535). It can exsit as a dimer(PMID:16919242).

Protocols

Product Specific Protocols
IF protocol for CL594 ALDH3A1 antibody CL594-68036Download protocol
FC protocol for CL594 ALDH3A1 antibody CL594-68036Download protocol
Standard Protocols
Click here to view our Standard Protocols