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ALDH5A1 Monoclonal antibody

ALDH5A1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1B9H10

Cat no : 67347-1-Ig

Synonyms

Succinate-semialdehyde dehydrogenase, mitochondrial, SSDH, SSADH, NAD(+)-dependent succinic semialdehyde dehydrogenase, EC:1.2.1.24


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Tested Applications

Positive WB detected inL02 cells, HepG2 cells, pig brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67347-1-Ig targets ALDH5A1 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ALDH5A1 fusion protein Ag11155 相同性解析による交差性が予測される生物種
Full Name aldehyde dehydrogenase 5 family, member A1
Calculated molecular weight 535 aa, 57 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC034321
Gene symbol ALDH5A1
Gene ID (NCBI) 7915
RRIDAB_2882605
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ALDH5A1 antibody 67347-1-IgDownload protocol
IHC protocol for ALDH5A1 antibody 67347-1-IgDownload protocol
IF protocol for ALDH5A1 antibody 67347-1-IgDownload protocol
FC protocol for ALDH5A1 antibody 67347-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols