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ALDH5A1 Monoclonal antibody
ALDH5A1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, IF-P, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
1B9H10
Cat no : 67347-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | L02 cells, HepG2 cells, pig brain tissue |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human liver cancer tissue |
Positive FC (Intra) detected in | A431 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
67347-1-Ig targets ALDH5A1 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | ALDH5A1 fusion protein Ag11155 相同性解析による交差性が予測される生物種 |
Full Name | aldehyde dehydrogenase 5 family, member A1 |
Calculated molecular weight | 535 aa, 57 kDa |
Observed molecular weight | 51 kDa |
GenBank accession number | BC034321 |
Gene symbol | ALDH5A1 |
Gene ID (NCBI) | 7915 |
RRID | AB_2882605 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for ALDH5A1 antibody 67347-1-Ig | Download protocol |
IHC protocol for ALDH5A1 antibody 67347-1-Ig | Download protocol |
IF protocol for ALDH5A1 antibody 67347-1-Ig | Download protocol |
FC protocol for ALDH5A1 antibody 67347-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |