ALDH5A1 Monoclonal antibody, PBS Only

L02 cells were subjected to SDS PAGE followed by western blot with 67347-1-Ig (ALDH5A1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67347-1-PBS in a different storage buffer formulation.

WB result of ALDH5A1 antibody (67347-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH5A1 transfected HepG2 cells. This data was developed using the same antibody clone with 67347-1-PBS in a different storage buffer formulation.

HepG2 cells were subjected to SDS PAGE followed by western blot with 67347-1-Ig (ALDH5A1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67347-1-PBS in a different storage buffer formulation.

pig brain tissue were subjected to SDS PAGE followed by western blot with 67347-1-Ig (ALDH5A1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67347-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67347-1-Ig (ALDH5A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67347-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67347-1-Ig (ALDH5A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67347-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67347-1-Ig (ALDH5A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67347-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67347-1-Ig (ALDH5A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67347-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ALDH5A1 antibody (67347-1-Ig, Clone: 1B9H10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67347-1-PBS in a different storage buffer formulation.

1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human ALDH5A1 (67347-1-Ig, Clone:1B9H10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67347-1-PBS in a different storage buffer formulation.