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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ALKBH3 Recombinant antibody, PBS Only (Capture)

ALKBH3 Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241650F10

Cat no : 84314-2-PBS

Synonyms

DEPC-1, DEPC1, DEPC 1, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, ABH3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ALKBH3 Recombinant antibody (84314-2-RR)

    WB analysis using 84314-2-RR (same clone as 84314-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84314-2-RR (ALKBH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84314-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ALKBH3 Recombinant antibody (84314-2-RR)

    WB analysis using 84314-2-RR (same clone as 84314-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84314-2-RR (ALKBH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84314-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01211-2

    Sandwich ELISA standard curve of MP01211-2

    Sandwich ELISA standard curve of MP01211-2, Human ALKBH3 Recombinant Matched Antibody Pair - PBS only. 84314-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2982. 84314-4-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Sample test of MP01211-2

    Sample test of MP01211-2

    The mean ALKBH3 concentration was determined to be 6.80 ng/mL in Jurkat cell extract based on a 1.50 mg/mL extract load.

  • Flow cytometry (FC) experiment of HeLa cells using ALKBH3 Recombinant antibody (84314-2-RR)

    FC experiment of HeLa using 84314-2-RR (same clone as 84314-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ALKBH3 Recombinant antibody (84314-2-RR, Clone:241650F10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84314-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of PC-3 cells using ALKBH3 Recombinant antibody (84314-2-RR)

    FC experiment of PC-3 using 84314-2-RR (same clone as 84314-2-PBS)

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug ALKBH3 Recombinant antibody (84314-2-RR, Clone:241650F10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84314-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01211-1

    Cytometric bead array standard curve of MP01211-1

    Cytometric bead array standard curve of MP01211-1, ALKBH3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84314-2-PBS. Detection antibody: 84314-1-PBS. Standard: Ag2982. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84314-2-RR

    Affinity and Kinetic Characterization of 84314-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84314-2-RR against Human ALKBH3 were performed. The affinity constant is 1.99 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84314-2-PBS targets ALKBH3 as part of a matched antibody pair:

MP01211-1: 84314-2-PBS capture and 84314-1-PBS detection (validated in Cytometric bead array)

MP01211-2: 84314-2-PBS capture and 84314-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ALKBH3 fusion protein Ag2982 相同性解析による交差性が予測される生物種
Full Name alkB, alkylation repair homolog 3 (E. coli)
Calculated molecular weight 286 aa, 33 kDa
Observed molecular weight30-33 kDa
GenBank accession numberBC015155
Gene symbol ALKBH3
Gene ID (NCBI) 221120
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ALKBH3, also named as ABH3 and DEPC-1, is a dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). It has a strong preference for single-stranded. ALKBH3 is requires molecular oxygen, alpha-ketoglutarate and iron. This gene has some isoforms with MW 19-25 kDa and 33 kDa.