Validation Data Gallery
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Tested Applications
| Positive WB detected in | A375 cells, mouse kidney tissue, SGC-7901 cells, mouse heart tissue, rat heart tissue |
| Positive FC (Intra) detected in | PC-3 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84314-2-RR targets ALKBH3 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | ALKBH3 fusion protein Ag2982 相同性解析による交差性が予測される生物種 |
| Full Name | alkB, alkylation repair homolog 3 (E. coli) |
| Calculated molecular weight | 286 aa, 33 kDa |
| Observed molecular weight | 30-33 kDa |
| GenBank accession number | BC015155 |
| Gene Symbol | ALKBH3 |
| Gene ID (NCBI) | 221120 |
| RRID | AB_3671855 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96Q83 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ALKBH3, also named as ABH3 and DEPC-1, is a dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). It has a strong preference for single-stranded. ALKBH3 is requires molecular oxygen, alpha-ketoglutarate and iron. This gene has some isoforms with MW 19-25 kDa and 33 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ALKBH3 antibody 84314-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |