Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HEK-293T |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:9000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
24550-1-AP targets AMOT in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | AMOT fusion protein Ag19784 相同性解析による交差性が予測される生物種 |
| Full Name | angiomotin |
| Calculated molecular weight | 1084 aa, 118 kDa |
| Observed molecular weight | 80 kDa, 130 kDa |
| GenBank accession number | BC130294 |
| Gene Symbol | AMOT |
| Gene ID (NCBI) | 154796 |
| RRID | AB_2879603 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q4VCS5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Angiomotin belongs to the motion family of angiostatin-binding proteins. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells during the formation of new blood vessels. The most abundant expression was found in the placenta and skeletal muscle. AMOT has two isoforms with MW 130 kDa (p130) and 80 kDa (p80). The p130 isoform can interact with F-actin. This antibody recognizes both p130 and p80.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AMOT antibody 24550-1-AP | Download protocol |
| IHC protocol for AMOT antibody 24550-1-AP | Download protocol |
| IF protocol for AMOT antibody 24550-1-AP | Download protocol |
| IP protocol for AMOT antibody 24550-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Theranostics The WW domains dictate isoform-specific regulation of YAP1 stability and pancreatic cancer cell malignancy. | ||
Sci Rep MAGI1 inhibits the AMOTL2/p38 stress pathway and prevents luminal breast tumorigenesis. | ||