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AMOT Polyclonal antibody

AMOT Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 24550-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

KIAA1071, Angiomotin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHEK-293 cells, HEK-293T
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:9000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24550-1-AP targets AMOT in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AMOT fusion protein Ag19784 相同性解析による交差性が予測される生物種
Full Name angiomotin
Calculated molecular weight 1084 aa, 118 kDa
Observed molecular weight 80 kDa, 130 kDa
GenBank accession numberBC130294
Gene Symbol AMOT
Gene ID (NCBI) 154796
RRIDAB_2879603
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ4VCS5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Angiomotin belongs to the motion family of angiostatin-binding proteins. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells during the formation of new blood vessels. The most abundant expression was found in the placenta and skeletal muscle. AMOT has two isoforms with MW 130 kDa (p130) and 80 kDa (p80). The p130 isoform can interact with F-actin. This antibody recognizes both p130 and p80.

Protocols

Product Specific Protocols
WB protocol for AMOT antibody 24550-1-APDownload protocol
IHC protocol for AMOT antibody 24550-1-APDownload protocol
IF protocol for AMOT antibody 24550-1-APDownload protocol
IP protocol for AMOT antibody 24550-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Theranostics

The WW domains dictate isoform-specific regulation of YAP1 stability and pancreatic cancer cell malignancy.

Authors - Qiang Guo
humanIF

Sci Rep

MAGI1 inhibits the AMOTL2/p38 stress pathway and prevents luminal breast tumorigenesis.

Authors - Diala Kantar
humanIP

PLoS One

Regulation of YAP and Wnt signaling by the endosomal protein MAMDC4

Authors - Christopher M Cox
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