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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated AMOT Polyclonal antibody

AMOT Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-24550

Synonyms

KIAA1071, Angiomotin

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CoraLite® Plus 488-conjugated AMOT Polyclonal anti (CL488-24550)

    IF Staining of HEK-293 using CL488-24550

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 AMOT antibody (CL488-24550) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-24550 targets AMOT in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AMOT fusion protein Ag19784 相同性解析による交差性が予測される生物種
Full Name angiomotin
Calculated molecular weight 1084 aa, 118 kDa
Observed molecular weight 80 kDa, 130 kDa
GenBank accession numberBC130294
Gene symbol AMOT
Gene ID (NCBI) 154796
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Angiomotin belongs to the motin family of angiostatin binding protein. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells during the formation of new blood vessels. Most abundant expression was found in placenta and skeletal muscle. AMOT has two isoforms with MW 130 kDa (p130) and 80 kDa (p80). The p130 isoform can interact with F-actin.This antibody recognizes both p130 and p80.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 AMOT antibody CL488-24550Download protocol
Standard Protocols
Click here to view our Standard Protocols