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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AMOTL2 Monoclonal antibody

AMOTL2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2C4A4

Cat no : 68401-1-Ig

Synonyms

AMOTL2, angiomotin like 2, Angiomotin like protein 2, KIAA0989, LCCP, Leman coiled coil protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AMOTL2 Monoclonal antibody (68401-1-Ig)

    WB analysis using 68401-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68401-1-Ig (AMOTL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of DU 145 cells using AMOTL2 Monoclonal antibody (68401-1-Ig)

    WB analysis of DU 145 using 68401-1-Ig

    DU 145 cells were subjected to SDS PAGE followed by western blot with 68401-1-Ig (AMOTL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Saos-2 cells using AMOTL2 Monoclonal antibody (68401-1-Ig)

    WB analysis of Saos-2 using 68401-1-Ig

    Saos-2 cells were subjected to SDS PAGE followed by western blot with 68401-1-Ig (AMOTL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NCI-H1299 cells using AMOTL2 Monoclonal antibody (68401-1-Ig)

    WB analysis of NCI-H1299 using 68401-1-Ig

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 68401-1-Ig (AMOTL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using AMOTL2 Monoclonal antibody (68401-1-Ig)

    IF Staining of A549 using 68401-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using AMOTL2 antibody (68401-1-Ig, Clone: 2C4A4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inA549 cells, DU 145 cells, Saos-2 cells, NCI-H1299 cells, MDA-MB-468 cells, SK-BR-3 cells
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68401-1-Ig targets AMOTL2 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AMOTL2 fusion protein Ag19971 相同性解析による交差性が予測される生物種
Full Name angiomotin like 2
Calculated molecular weight 779 aa, 86 kDa
Observed molecular weight100 kDa
GenBank accession numberBC011454
Gene symbol AMOTL2
Gene ID (NCBI) 51421
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Angiomotin‑like 2 (AMOTL2) is a member of the motin family of angiostatin‑binding proteins. The motin family, also known as AMOTs, consists of three members: AMOT, AMOT-like 1 (AMOTL1) and AMOTL2. Members of the motin family are a type of adaptor proteins mainly distributed in the cytomembrane, cytoplasm or nucleus, and have a higher expression in the endothelial cells of capillaries and in larger vessels of the placenta. Human AmotL2 encodes two isoforms of a molecular mass of 100 kDa and 60 kDa.(PMID: 34036399,PMID: 25080976)

Protocols

Product Specific Protocols
WB protocol for AMOTL2 antibody 68401-1-IgDownload protocol
IF protocol for AMOTL2 antibody 68401-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols