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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated AMOTL2 Monoclonal antibody

AMOTL2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C4A4

Cat no : CL488-68401

Synonyms

AMOTL2, angiomotin like 2, Angiomotin like protein 2, KIAA0989, LCCP, Leman coiled coil protein

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CoraLite® Plus 488-conjugated AMOTL2 Monoclonal an (CL488-68401)

    IF Staining of A549 using CL488-68401

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 AMOTL2 antibody (CL488-68401, Clone: 2C4A4 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68401 targets AMOTL2 in IF/ICC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AMOTL2 fusion protein Ag19971 相同性解析による交差性が予測される生物種
Full Name angiomotin like 2
Calculated molecular weight 779 aa, 86 kDa
Observed molecular weight100 kDa
GenBank accession numberBC011454
Gene symbol AMOTL2
Gene ID (NCBI) 51421
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Angiomotin‑like 2 (AMOTL2) is a member of the motin family of angiostatin‑binding proteins. The motin family, also known as AMOTs, consists of three members: AMOT, AMOT-like 1 (AMOTL1) and AMOTL2. Members of the motin family are a type of adaptor proteins mainly distributed in the cytomembrane, cytoplasm or nucleus, and have a higher expression in the endothelial cells of capillaries and in larger vessels of the placenta. Human AmotL2 encodes two isoforms of a molecular mass of 100 kDa and 60 kDa.(PMID: 34036399,PMID: 25080976)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 AMOTL2 antibody CL488-68401Download protocol
Standard Protocols
Click here to view our Standard Protocols